Adventure Sports

How should I measure the survival rate of the bacteria serratia marcesens?

I’m doing a science research project where I am measuring the survival rate of the bacteria serratia marcesens on 3 different surfaces- copper, stainless steel, and copper alloy. At different time intervals, the amount of bacteria on each surface has to somehow be measured, but I am having a difficult time coming up with an experimental design. Any ideas?

Typically, plated cultures are “measured” by the number of individual colonies present, and/or their diameter.

I assume you will start with a liquid innoculum. If you use the same liquid innoculum, and you pipette a known amount of liquid onto your particular surface, spread it, and count how many colonies (and their size just for anecdotal data) after a given period of time, this will give a good comparative basis to see how the bacteria survive under the particular conditions. Don’t forget to include a control plate (a simple LB agar plate should suffice).

Hope I’ve helped!

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